Diversity of Entomopathogenic Fungi in Argane Forest Soil and Their Potential to Manage Mediterranean Fruit Fly (Ceratitis capitata)
A.Hallouti , A. Zahidi , R. Bouharroud , A. El Mousadik , A. Ait Ben Aoumar and H. Boubaker
Journal of Pharmacy and Pharmacology 5 (2017) 746-754
doi: 10.17265/2328-2150/2017.010.007
Abstract: The
present study forms a part of biological control against the medfly (Ceratitis
capitata) and seeks to isolate and determine the diversity of the
medfly-associated entomopathogenic fungi in soil samples collected from Argane
(Argania spinosa) forest, in different localities of Souss-Massa region. Search
and isolation of the indigenous populations of Ceratitis capitata
entomopathogenic fungi was carried out by using larvae of this pest as baits.
After trapping, isolation and identification of fungal isolates, a
pathogenicity test was used to select fungal strains that have significant
virulence potential against the Mediterranean fruit fly. The degree of
virulence was estimated by the ability of the fungus to induce the disease
and/or the death in the insect and also by calculating the lethal time 50
(LT50). The obtained results were clearly demonstrated the sensitivity of
medfly to tested fungal strains and particularly to strains of Fusarium sp.,
Aspergillus niger and Scopulariopsis sp. that have shown high mortality rates
(more than 84%) and to Trichoderma harzianum, Scedosporium sp., Epicoccum sp.
and Ulocladium sp. with more than 70% mortality for the two tested
concentrations. Furthermore these strains showed short LT50 (less than 83
hours). All these results confirm the presence of entomopathogenic fungi of
Ceratitis capitata in Argane soil, and prove the potential of entomopathogenic
fungi for biocontrol of the Mediterranean fruit fly under laboratory
conditions.
Key words:
Biological control, Ceratitis capitata, entomopathogenic fungi, Argania
spinosa.
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