Ressources génétiques/Olivier1

Fingerprinting of the main olive cultivars in Tunisia by morphological and AFLP markers. 

S.R. MNASRI, O.D SADDOUD, S. ROUZ, M. BEN SALEH, A. FERCHICHI

Volume 37(6). Published January, 01, 2017

Abstract - A study was conducted to compare morphological and genetical data of the main local olive cultivars in Tunisia. This work was conducted in the framework of the activities of the fruit tree network in the Tunisian National Gene Bank. Samples were taken from leaves, fruits and stones for morphological characters. DNA was extracted from leaf tissue and 6 EcoRI–MseI AFLP primer combinations were used. The morphological study permitted a specific description of the characteristics for the tested varieties and their repartition into three groups according to the fruit and endocarp quantitative data. Molecular data analysis demonstrated a high degree of polymorphism with an average of 35%. The analysis of AFLP profiles found in our set of olive cultivars showed a wide genetic diversity among olive germplasm. The UPGMA cluster analyses using Jaccard’s index revealed that the genetic diversity was predominantly structured according to fruit size. The data obtained was used for the varietal survey and construction of National Gene Bank fruit crops database, which will help in providing also additional information that could form the basis for the national design of olive breeding programs.

Keywords: AFLP, Gene bank, local cultivar olive, morphological data

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